Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies


  • Saima Nadeem Assistant Professor Histopathology, Khyber Girls Medical College, Peshawar
  • Nuzhat Sultana Department of Pathology, Northwest Medical College, Peshawar
  • Shazia Naz Department of Pathology, Kabir Medical College, Peshawar
  • Momena Ali Department of Pathology, Khyber Medical College, Peshawar
  • Amina Gul Department of Pathology, Khyber Medical College, Peshawar, Pakistan
  • Ambareen Moatassim PAEC Hospital, Islamabad


Giemsa Stain, Predictive values, Immunohistochemical stain, Helicobacter Pylori


Objective: To compare the efficacy of different staining (H&E, modified Giemsa stain and Immunohistochemical stains) for detecting H pylori in gastric biopsies.

Methodology: This descriptive study was carried out at the Department of Pathology, Khyber Girls Medical College/ Hayatabad Medical Complex Peshawar. A non-probability convenience sampling technique was used to select study participants from December 2018 to July 2019. Total 104 gastric biopsies obtained through upper GI endoscopy were processed for identification of H pylori through various staining techniques. All the participants consented to participate in the study. The study was conducted after approval by institutional ethical committee.

Results: Among total 104 cases analyzed, 63 cases were found positive for h pylori infection using H and E stain. Based on Immunohistochemical analysis 101 turned out to be infected with H pylori. The third stain used for H pylori detection was Giemsa stain which revealed the highest percentage of H pylori positivity (100%) after IHC stain. All the 104 cases analyzed were found positive on Giemsa stain.

Conclusion: In daily practice modified Giemsa stain may be sufficient for diagnosis of H.pylori organism






Original article

How to Cite

Nadeem S. Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies. Int J Pathol [Internet]. 2021 Aug. 23 [cited 2024 Jun. 14];19(1):28-31. Available from:

Most read articles by the same author(s)

1 2 > >>