Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies

Authors

  • Saima Nadeem Assistant Professor Histopathology, Khyber Girls Medical College, Peshawar
  • Nuzhat Sultana Department of Pathology, Northwest Medical College, Peshawar
  • Shazia Naz Department of Pathology, Kabir Medical College, Peshawar
  • Momena Ali Department of Pathology, Khyber Medical College, Peshawar
  • Amina Gul Department of Pathology, Khyber Medical College, Peshawar, Pakistan
  • Ambareen Moatassim PAEC Hospital, Islamabad

Keywords:

Giemsa Stain, Predictive values, Immunohistochemical stain, Helicobacter Pylori

Abstract

Objective: To compare the efficacy of different staining (H&E, modified Giemsa stain and Immunohistochemical stains) for detecting H pylori in gastric biopsies.

Methodology: This descriptive study was carried out at the Department of Pathology, Khyber Girls Medical College/ Hayatabad Medical Complex Peshawar. A non-probability convenience sampling technique was used to select study participants from December 2018 to July 2019. Total 104 gastric biopsies obtained through upper GI endoscopy were processed for identification of H pylori through various staining techniques. All the participants consented to participate in the study. The study was conducted after approval by institutional ethical committee.

Results: Among total 104 cases analyzed, 63 cases were found positive for h pylori infection using H and E stain. Based on Immunohistochemical analysis 101 turned out to be infected with H pylori. The third stain used for H pylori detection was Giemsa stain which revealed the highest percentage of H pylori positivity (100%) after IHC stain. All the 104 cases analyzed were found positive on Giemsa stain.

Conclusion: In daily practice modified Giemsa stain may be sufficient for diagnosis of H.pylori organism

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Published

2021-08-23

Issue

Volume.19, Issue-1 (Jan-Mar 2021)

Section

Original article

License

Copyright (c) 2021 Saima Nadeem; Nuzhat Sultana, Shazia Naz, Momena Ali, Amina Gul, Ambareen Moatassim

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Readers may “Share-copy and redistribute the material in any medium or format” and “Adapt-remix, transform, and build upon the material”. The readers must give appropriate credit to the source of the material and indicate if changes were made to the material. Readers may not use the material for commercial purpose. The readers may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

How to Cite

1.
Nadeem S. Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies. Int J Pathol [Internet]. 2021 Aug. 23 [cited 2024 Dec. 25];19(1):28-31. Available from: https://jpathology.com/index.php/OJS/article/view/635

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