Hygienic production practices and microbial evaluation of cow milk quality in District Gilgit

Authors

  • Ishrat Roomi Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan
  • Azhar Hussain Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan
  • Sartaj Khalil Government of Gilgit-Baltistan
  • Shahnawaz Laqdoom Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan
  • Waqar Younus Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan
  • Ghulam Abbas Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

DOI:

https://doi.org/10.59736/IJP.22.02.893

Keywords:

Raw milk, microbial quality, hygiene practices, , Total Bacterial Count, Total Coliform Count, , Methylene Blue Reduction Test.

Abstract

Background: Dairy cow’s raw milk may get contaminated by pathogens released by diseased animals, bacteria from the environment, or germs from the udder. Insufficient hygiene on the udder and teat surfaces, as well as contaminated milking equipment, can transfer environmental organisms into the milk.  

Methods: To examine the practices of hygienic cow milk production, a study was conducted with 175 respondents from District Gilgit, who were selected to evaluate their understanding and implementation of hygienic milk production methods. The participants were interviewed to determine their level of understanding. In addition, fifty milk samples were collected from the producers to assess the microbiological purity of the milk. 

Results: The findings revealed that a significant proportion of milk producers neglected or were unaware of essential hygiene practices, such as washing hands, udders/teats of cows, and milking utensils before milking. The predominant water sources used were tap and stream, with the barn conditions largely being dusty despite daily cleaning efforts by most respondents. It was observed that milk processing and refrigeration post-milking were common practices, though a minority reported using injections and supplements preceding milking activities. The quality of milk was evaluated using the Methylene Blue Reduction Test (MBRT), Total Bacterial Count (TBC) and Total Coliform Count (TCC). Analytical results indicated MBRT durations ranging from 1 to 8.5 hours, with TBC and TCC levels extending from 2.16x106 to 1.008x107 and 3x105 to 6.56x106, respectively. Most of the milk samples were of fair quality having average MBR time and the TBC and TCC have values above the upper limits set to 105.

Conclusion: The finding identified inadequate hygiene, low water quality for cleaning, and poor barn conditions as the primary factors contributing to the low milk quality in Gilgit.  Therefore, it is essential to enhance the hygienic dairy practices and promote public health.

Author Biographies

  • Ishrat Roomi, Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

    Department of Forestry, Range and Wildlife Management

  • Azhar Hussain, Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

    Department of Agriculture and Food Technology

  • Sartaj Khalil, Government of Gilgit-Baltistan

    Department of Health

  • Shahnawaz Laqdoom, Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

    Department of Agriculture and Food Technology

  • Waqar Younus, Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

    Department of Agriculture and Food Technology

  • Ghulam Abbas, Karakoram International University, Gilgit, Gilgit-Baltistan, Pakistan

    Department of Agriculture and Food Technology

References

References

Muhammad K, Altaf I, Hanif A, Anjum AA, Tipu MY. Monitoring of hygienic status of raw milk marketed in Lahore city, Pakistan. Journal of Animal & Plant Sciences. 2009; 19(2):74-77.

Reta MA, Addis AH. Microbiological quality assessment of raw and pasteurized milk. International Journal of Food Science and Microbiology. 2015; 2(6):087-91.

Marjan S, Kanta Das K, Kishore Munshi S, Noor R. Drug-resistant bacterial pathogens in milk and some milk products. Nutrition & Food Science. 2014 May 6; 44(3):241-8.

Ensminger M E. Milk Secretion and Handling in Dairy Cattle Science, 3rd Edition, Interstate Publishing, Inc., Danville, Illinois. 1993; 416-422.

Ashenafi M. Microbiological quality of ayib, a traditional Ethiopian cottage cheese. International Journal of Food microbiology. 1990; 10(3-4):263-268.

Negash F, Tadesse E, Woldu T. Microbial quality and chemical composition of raw milk in the Mid-Rift Valley of Ethiopia. African Journal of Agricultural Research. 2012 Jul 31; 7(29):4167-70.

Asaminew T, Eyassu S. 2011. Microbial quality of raw cow's milk collected from farmers and dairy cooperatives in Bahir Dar Zuria and Mecha district, Ethiopia. Agriculture and Biology Journal of North America. 2011; 2(1):29-33.

Tola A, Ofodile LN, Beyene F. Microbial quality and chemical composition of raw whole milk from Horro cattle in East Wollega, Ethiopia. Ethiopian Journal of Education and Sciences. 2007; 3(1):1-0.

Soomro AH, Arain MA, Khaskheli M, Bhutto B. Isolation of Escherichia coli from raw milk and milk products in relation to public health sold under market conditions at Tandojam. Pakistan Journal of Nutrition. 2002; 1(3):151-2.

Teka G. Food hygiene principles and food borne disease control with special reference to Ethiopia. Faculty of Veterinary Medicine, Department of Community Health, Addis Ababa University. 1997.

Oliver SP, Jayarao BM, Almeida RA. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathogens & Disease. 2005 Jun 1; 2(2):115-29.

Marjan S, Kanta Das K, Kishore Munshi S, Noor R. Drug-resistant bacterial pathogens in milk and some milk products. Nutrition & Food Science. 2014 May 6; 44(3):241-8.

Torkar KG, Teger SG. The microbiological quality of raw milk after introducing the two day's milk collecting system. Acta Agriculturae Slovenica. 2008 Nov 10; 92(1):61-74.

Bereda A, Yilma Z, Nurfeta A. Hygienic and microbial quality of raw whole cow’s milk produced in Ezha district of the Gurage zone, Southern Ethiopia. Wudpecker Journal of Agricultural Research. 2012 Dec; 1(11):459-65.

Kuma A, Abdisa M, Tolossa D. Evaluation of hygienic status and marketing system of raw cow milk in different critical points of Oromia special zone. Global Journal of Science Frontier Research: C Biological Science. 2015; 15(4):21-30.

Yilma Z. Microbial properties of Ethiopian marketed milk and milk products and associated critical points of contamination: An epidemiological perspective. Epidemiology insights. 2012 Apr 20; 15:298-322.

ISO 6730. Milk–Enumeration of colony-forming units of psychrotrophic microorganisms – Colony-count technique at 6.5 degrees C. International Organization for Standardization, Genova, Switzerland. 2005; 1–6.

Collins CH, Lyne PM and Grange JM. Counting Microorganisms In “Microbiological Methods”, 6th Edition, Butterworth/Heinemann. 1989; 127-140.

Benson JH. Microbiological applications: Laboratory manual in general Microbiology, 8th Edition. 2002; 1-478.

Depiazzi L, Bell I. Effects of pre-milking teat sanitation on the quality of raw milk. Department of Primary Industries and Regional Development, Western Australia, Perth. Bulletin. 2002; 4563. https://library.dpird.wa.gov.au/bulletins/185

Welearegay H, Yilma Z, Tekle-Giorgis Y. Hygienic practices and microbiological quality of raw milk produced under different farm size in Hawassa, southern Ethiopia. Agricultural Research and Review. 2012; 1(4):1132-42.

Ruegg PL. The role of hygiene in efficient milking. WCDS advances in Dairy Technology. 2006; 18:285-93.

Getachew F. A Review of the small scale dairy sector in Ethiopia. FAO prevention of food losses programme. milk and milk products, post-harvest losses and food safety in sub-saharan Africa and Near East. 2003.

Yilma Z. Microbial properties of Ethiopian marketed milk and milk products and associated critical points of contamination: An epidemiological perspective. Epidemiology insights. 2012 Apr 20; 15:298-322.

Gurmessa T. Physicochemical Properties and Microbial Quality of Raw Cow’s Milk in Yabello District, Borana Zone, Southern Ethiopia. MSc Thesis, Haramaya University, Haramaya. 2014.

Ruegg PL. The role of hygiene in efficient milking. WCDS advances in Dairy Technology. 2006; 18:285-93.

MUIR DD. The shelf‐life of dairy products: 2. Raw milk and fresh products. International Journal of Dairy Technology. 1996 May; 49(2):44-8.

Asaminew T. Production, handling, traditional processing practices and quality of milk in Bahir Dar milk shed area, Ethiopia. M.Sc. Thesis, Haramaya University of Agriculture, Dire Dawa, Ethiopia. 2007.

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Published

2024-09-15

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Section

Original article

How to Cite

1.
Ishrat Roomi, Azhar Hussain, Sartaj Khalil, Shahnawaz Laqdoom, Waqar Younus, Ghulam Abbas. Hygienic production practices and microbial evaluation of cow milk quality in District Gilgit. Int J Pathol [Internet]. 2024 Sep. 15 [cited 2024 Nov. 21];22(2):46-54. Available from: https://jpathology.com/index.php/OJS/article/view/893