Frequency and antimicrobial susceptibility profile of Methicillin-resistant Staphylococcus aureus isolated from Pus and Swab samples in a Teaching Hospital of Lahore

Authors

  • Tariq Mahmud Tariq Shalamar Institute of Health Sciences, Lahore
  • Kainat Anwar Shalamar School of Allied Health Sciences, Lahore
  • Mariam Danish Iqbal Shalamar Institute of Health Sciences, Lahore
  • Sana Fatima Dogar
  • Naureen Saeed , Shalamar Institute of Health Sciences, Lahore

DOI:

https://doi.org/10.59736/IJP.22.01.868

Keywords:

Frequency, MRSA, Pus, Staphylococcus, Swab

Abstract

Background: Staphylococcus is a ubiquitous bacterium and well-known pathogen causing a variety of infections. The global spread of MRSA constitutes one of the most prevailing challenges to the management of infections caused by this bug.

Aim: To determine the frequency of MRSA and antibiogram profile of S. aureus recovered from various clinical samples.

Setting: Microbiology Laboratory of Shalamar Hospital Lahore.

Methods: The data of the Staphylococcus isolates including MRSA from various pus and swab samples was collected through Electronic Medical Record of the Shalamar Hospital from 1st Jan to 31st Dec 2021. S. aureus was identified by standard protocol including Gram stain, catalase, coagulase, and DNase tests. Antimicrobial susceptibility was carried out by modified Kirby Bauer method. MRSA frequency was determined by the result of sensitivity to cefoxitin.

Results: Out of 885 samples submitted for culture, 517 (58.4%) were reported for microbial growth of a known pathogen. The most frequently isolated pathogen was S. aureus (37.9%), followed by E. coli (22.4%), other members of Enterobacteriaceae family (17.8%), Pseudomonas (15.5%), Enterococcus (3.5%), Candida (2.1%), and Streptococcus (0.8%). Amongst S. aureus, MRSA was documented in 46.9% cases. Substantial difference was detected in the susceptibility pattern of methicillin-sensitive and resistant staphylococci. All staphylococci were resistant to ampicillin while no vancomycin resistance was encountered.

Conclusion: MRSA was seen in the local population with a high frequency of 46.9% and such MRSA also showed marked resistance against other commonly used antibiotics. Fortunately no vancomycin-resistant S. aureus was reported.

Author Biographies

  • Kainat Anwar, Shalamar School of Allied Health Sciences, Lahore

    Medical Laboratory Technologist

  • Mariam Danish Iqbal, Shalamar Institute of Health Sciences, Lahore

    Assistant Professor Microbiology, Department of Pthology, Shalamar Medical & Dental College Lahore

  • Sana Fatima Dogar

    Senior Demonstrator Pathology, Shalamar Medical & Dental College, Lahore

  • Naureen Saeed, , Shalamar Institute of Health Sciences, Lahore

    Associate Professor Haematology, Department of Pathology, Shalamar Medical & Dental College Lahore

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Published

2024-04-08

Issue

Volume.22, Issue-1 (Jan-Mar 2024)

Section

Original article

License

Copyright (c) 1970 Tariq Mahmud Tariq, Kainat Anwar, Mariam Danish Iqbal, Sana Fatima Dogar, Naureen Saeed

Creative Commons License

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Readers may “Share-copy and redistribute the material in any medium or format” and “Adapt-remix, transform, and build upon the material”. The readers must give appropriate credit to the source of the material and indicate if changes were made to the material. Readers may not use the material for commercial purpose. The readers may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

How to Cite

1.
Tariq TM, Anwar K, Iqbal MD, Dogar SF, Saeed N. Frequency and antimicrobial susceptibility profile of Methicillin-resistant Staphylococcus aureus isolated from Pus and Swab samples in a Teaching Hospital of Lahore. Int J Pathol [Internet]. 2024 Apr. 8 [cited 2024 May 1];22(1):24-9. Available from: https://jpathology.com/index.php/OJS/article/view/868