Detection of Immunoglobulin Gene Rearrangement in Acute Lymphoblastic Leukemia by Polymerase Chain Reaction
Keywords:
Ig gene rearrangement, ALL, PCRAbstract
Background: The objective of the study was to evaluate a qualitative PCR based method for detection of clonal
immunoglobulin gene rearrangement in patients of acute lymphoblastic leukemia. It was a descriptive Cross sectional
and it was conducted at Armed Forces Institute of Pathology Rawalpindi between Aug 2009- Jan2010.
Method: 61 patients with acute lymphoblastic leukemia were studied. Genomic DNA was extracted from the
peripheral blood, bone marrow aspirate/unstained bone marrow smears. PCR amplification of the IgH gene was
done by a VH primer homologous with a highly conserved sequence near the 3’ end of the FR3 region and a consensus
sequence JH primer. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel.
Results: A sharp clonal band ranging from 90-200 bp indicated a positive reaction. At the time of diagnosis 50
out of the 61 patients (82%) showed clonal immunoglobulin gene rearrangement by qualitative PCR using a single
pair of primers. Eleven (18%) patients did not show the Ig gene rearrangement.
Conclusion: Clonal Ig gene rearrangements can be demonstrated in a vast majority of patients of Acute Lymphoblastic
Leukemia by simple PCR using a single pair of primers.
Downloads
Published
Issue
Section
License
Readers may “Share-copy and redistribute the material in any medium or format” and “Adapt-remix, transform, and build upon the material”. The readers must give appropriate credit to the source of the material and indicate if changes were made to the material. Readers may not use the material for commercial purpose. The readers may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
