Comparison of 5 cc and 20 cc Syringes on FNAC Yield of Spermatogenic cells of Goat Testis

Abstract

Objective: To compare yield of spermatogenic cells on Fine Needle Aspiration Cytology by 5 cc and 20 cc
syringes on goat testis
Type of Study: Prospective, animal experimental
Place of Study: Islamabad Institute of Pathology and Azad Jammu Kashmir Medical College Muzaffarabad Azad
Kashmir, Pakistan
Duration of Study: December 2011- January 2012
Material and Methods: Fine Needle Aspiration Cytology was performed on goat testis using 5 cc and 20 cc
syringes. Five slides were made for each of 5 cc and 20 cc syringes (Total 10 slides). 10 passes were made for each
aspirate of a slide. The slides were air dried and stained with Giemsa stain. Five optimal high dry (X400
magnification) fields on each slide were selected separately for spermatogoonia and spermatozoa. They were
counted in each of respective fields. After totalling of the counts, average counts of spermatogoonia and
spermatozoa per field were obtained on each slide. The average counts on five slides were then totaled and
divided by five to obtain average count per field per slide count. A total of 100 fields were thus counted (For
spermatogoonia and spermatozoa 25 separate fields (50 fields) for each of 5 cc and 20 cc syringes).
Results: The average counts per field per slide on 5 cc and 20 cc syringes respectively for spermatogoonia were
38.88 and 40.84; and for spermatozoa were 77.68 and 78.88. On cumulative 100 high power fields there was hardly
any difference between 5 cc syringe and 20 cc syringe yields. These are essentially the same results and even do
not require any statistical tests to prove their difference.
Conclusion: The yield of spermatogoonia and spermatozoa was essentially the same for 5 cc syringe and 20 cc
syringes. The increased vacuum negative pressure did not result in increased yield of spermatogoonia and
spermatozoa. 5 cc syringes which can be easily handled by one hand requiring no special instruments can bring