Mohammad Yousaf1, Sajjad Ahmad2, Adil Yousaf3, Asia Razza4, Asma Bibi5, Ibrahim6, Saleem Nawaz 7, Sahibzada Muhammad Jawad 8, Fiaza Javed9 and Riffat Jehan10
1,4,5,6,7,9Department of Chemistry Islamia college university Peshawar, 2Hayat Abad medical complex, Peshawar,3Khber College of Dentistry, Peshawar, 10Department of Chemistry Shaheed Benazir Bhutto Women University, Peshawar, 8Department of Zoology Islamia College University Peshawar.
Introduction: Trace elements are supposed to play a role in carbohydrate metabolism as reported by many researchers. A number of studies have reported that imbalance of certain trace metals cause diabetes and vice versa
Objective: The aim of the study was to compare serum level of Chromium (Cr) and Zinc (Zn) in young adult female type-1 and type-2 diabetic subjects treated with Combination insulin and Sitagliptin/Metformin combination therapy in Khyber Pakhtun Khwa Pakistan.
Methodology: The study population includes 124 type-1 and 137 type-2 female diabetic subjects aged 18-45 years (n=261) .Data from the study subjects regarding their age, weight, height, and medical history was collected on a well-designed proforma after their informed consent. Fresh samples of blood were taken from 261 female diabetic subjects in a Gel tube. HbA1c was determined on BIO-RAD VARIANT II TURBO using HbA1c Kit –2. Serum Zinc and Chromium were determined by atomic absorption spectrophotometer using acetylene flame (Model Perkin Elmer AAS 700) under standard conditions.
Results: The mean value of HbA1c of type-1 study population (10.62%) was higher as compared to the mean HbA1c of type-2 (8.32%). The mean level of serum Zn in type- I study population (0.04 mg/L) was higher than type-2 (0.03 mg/L) study population. The mean level of serum Cr in type-I study population (0.04 mg/L) was higher than type-2 study population (0.02 mg/L). Bivariate Pearson’s Correlations analysis of serum Zn (mg/L) with HbA1C (%) shows a highly significant correlation (ƿ=0.00).
Conclusion: The findings of this study suggest that treatment with insulin may be a better option in term of trace metal homeostasis in this selected group of patients.
Key words: Trace metals; Diabetes; Zinc; Homeostasis