Original Article: Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies

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Professor, Consultant Pathologist, American Board Certified Pathologist, Fellow College of American Pathologists. Areas of interest Fine Needle Aspiration Cytology, Cytology, Cancer, Ethics, Islam, Humanity

Original Article: Comparison of Different Staining Techniques for Identification of Helicobacter Pylori in Gastric Biopsies

1Saima Nadeem, 2Nuzhat Sultana, 3Shazia Naz, 4Momena Ali, 5Amina Gul and 6Ambareen Moatassim
1Department of Pathology, Khyber Girls Medical College, Peshawar, 2Department of Pathology, Northwest Medical College, Peshawar, 3Department of Pathology, Kabir Medical College, Peshawar, 4Department of Pathology, Khyber Medical College, Peshawar, 5Department of Pathology, Khyber Medical College, Peshawar, Pakistan, 6 PAEC Hospital, Islamabad, .

ABSTRACT
Objective: To compare the efficacy of different staining (H&E, modified Giemsa stain and Immunohistochemical stains) for detecting H pylori in gastric biopsies.
Methodology: This descriptive study was carried out at the Department of Pathology, Khyber Girls Medical College/ Hayatabad Medical Complex Peshawar. A non-probability convenience sampling technique was used to select study participants from December 2018 to July 2019. Total 104 gastric biopsies obtained through upper GI endoscopy were processed for identification of H pylori through various staining techniques. All the participants consented to participate in the study. The study was conducted after approval by institutional ethical committee.
Results: Among total 104 cases analyzed, 63 cases were found positive for h pylori infection using H and E stain. Based on Immunohistochemical analysis 101 turned out to be infected with H pylori. The third stain used for H pylori detection was Giemsa stain which revealed the highest percentage of H pylori positivity (100%) after IHC stain. All the 104 cases analyzed were found positive on Giemsa stain.
Conclusion: In daily practice modified Giemsa stain may be sufficient for diagnosis of H.pylori organism.



         

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