Evaluation of Anti Erythropoietin Antibodies in Chronic Renal Failure Patients Undergoing Haemodialysis
Introduction: Anemia is frequently seen in CKD patients on dialysis as a complication due to anti-EPO antibodies.
Objective: Present study was planned to detect the anti-EPO antibodies in CRF patients undergoing haemodialysis and study its correlation with baseline parameters such as Hb, retic count and iron markers (serum Iron, Ferritin, TIBC)
Study Design: Cross-sectional study. Setting: Haematology Department of University of health sciences Lahore in collaboration with the dialysis centre of Sheikh Zayed Hospital, Lahore.
Study Period: Six months from January to June 2016.
Materials & Methods: Total eighty six (86) patients, selected from haemodialysis centre of Sheikh Zayed Hospital Lahore were included in the study. Complete blood count, retic count, anti- EPO Antibodies, Serum Ferritin, Serum Iron, TIBC were performed after clinical history, physical exam and written consent. Data was analysed by using SPSS 20.0 version.
Results: Our results showed that 51 patients were positive for anti-EPO antibodies and 35 were negative for anti-EPO antibodies. In our study, these might be non-neutralizing antibodies because they did not have any correlation with Hb and reticulocyte count.
Independent sample t-test and Mann Whitney-U test applied, showed a statistically insignificant difference (p=>0.05) regarding baseline parameters (Hb, Hct, Urea and Creatinine) between positive and negative anti-EPO antibodies. Correlation analysis showed that anti-EPO antibodies did not significantly correlated with Hb, Hct, Urea, Creatinine, serum iron, ferritin, TSAT% and reticulocyte count. However, a significant correlation of anti-EPO antibodies (p=0.01) was observed only with TIBC but this was a negative correlation.
Conclusion: Our study shows that early detection of these antibodies is important to reduce morbidity and improve patient´s quality of life