Ayesha Nayyar* and Suhaib Ahmed**
* Islamic International Medical College, ** Armed Forces Institute of Pathology
Background: The objective of the study was to evaluate a qualitative PCR based method for detection of clonal
immunoglobulin gene rearrangement in patients of acute lymphoblastic leukemia. It was a descriptive Cross sectional
and it was conducted at Armed Forces Institute of Pathology Rawalpindi between Aug 2009- Jan2010.
Method: 61 patients with acute lymphoblastic leukemia were studied. Genomic DNA was extracted from the
peripheral blood, bone marrow aspirate/unstained bone marrow smears. PCR amplification of the IgH gene was
done by a VH primer homologous with a highly conserved sequence near the 3’ end of the FR3 region and a consensus
sequence JH primer. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel.
Results: A sharp clonal band ranging from 90-200 bp indicated a positive reaction. At the time of diagnosis 50
out of the 61 patients (82%) showed clonal immunoglobulin gene rearrangement by qualitative PCR using a single
pair of primers. Eleven (18%) patients did not show the Ig gene rearrangement.
Conclusion: Clonal Ig gene rearrangements can be demonstrated in a vast majority of patients of Acute Lymphoblastic
Leukemia by simple PCR using a single pair of primers.
Key words: Ig gene rearrangement; ALL; PCR