Detection of Immunoglobulin Gene Rearrangement in Acute Lymphoblastic Leukemia by Polymerase Chain Reaction

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Professor, Consultant Pathologist, American Board Certified Pathologist, Fellow College of American Pathologists. Areas of interest Fine Needle Aspiration Cytology, Cytology, Cancer, Ethics, Islam, Humanity

Detection of Immunoglobulin Gene Rearrangement in Acute Lymphoblastic Leukemia by Polymerase Chain Reaction

Ayesha Nayyar* and Suhaib Ahmed**
* Islamic International Medical College, ** Armed Forces Institute of Pathology

Background: The objective of the study was to evaluate a qualitative PCR based method for detection of clonal
immunoglobulin gene rearrangement in patients of acute lymphoblastic leukemia. It was a descriptive Cross sectional
and it was conducted at Armed Forces Institute of Pathology Rawalpindi between Aug 2009- Jan2010.
Method: 61 patients with acute lymphoblastic leukemia were studied. Genomic DNA was extracted from the
peripheral blood, bone marrow aspirate/unstained bone marrow smears. PCR amplification of the IgH gene was
done by a VH primer homologous with a highly conserved sequence near the 3’ end of the FR3 region and a consensus
sequence JH primer. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel.
Results: A sharp clonal band ranging from 90-200 bp indicated a positive reaction. At the time of diagnosis 50
out of the 61 patients (82%) showed clonal immunoglobulin gene rearrangement by qualitative PCR using a single
pair of primers. Eleven (18%) patients did not show the Ig gene rearrangement.
Conclusion: Clonal Ig gene rearrangements can be demonstrated in a vast majority of patients of Acute Lymphoblastic
Leukemia by simple PCR using a single pair of primers.
Key words: Ig gene rearrangement; ALL; PCR

         

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