Author Archives: mshafee

Frequency and antimicrobial sensitivity Pattern of Pseudomonas areuginosa from burn Patients in Lahore, Pakistan

Introduction: Burn injuries are more common in low income countries and are one of the most leading causes of increasing mortality in population. Increasing pattern of drug resistance has made it difficult to treat patients promptly. A descriptive study was planned to estimate the frequency and susceptibility pattern of Pseudomonas aeruginosa in burn patients and was carried out at the Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore during 2014. Materials & Methods: A total of 170 swab samples were collected from 90 indoor patients from burn wound infected patients from Mayo Hospital, Lahore, Pakistan. Samples were then subjected to culture on different microbiological media (MacConkey’s agar, Blood agar, Nutrient agar and Cetrimide agar) alongwith different biochemical tests. Antimicrobial sensitivity was done with disc diffusion procedure. Results: Revealed 90/170 (52.9%) samples were contaminated with Pseudomonas aeruginosa in 49 (54.45 %) male and 41 (45.56 %) female patients with 31.23 years mean age. The most common cause was flame burns 41(45.55 %), followed by scalds burns 22 (24.44 %), electric burns 15 (16.66 %) and acid burns 12 (13.33 %), respectively. A total 78 isolates of Pseudomonas aeruginosa were recovered from burn patients and most susceptible antibiotic were Imipenem 75.65 %, Cefatoxime 65.39 %, Tobramycin 64.11 % and Amikacin 60.26 % against it. Conclusion: Majority of burn patients were male aging 30-50 Years with flame burn main cause of accident and incident.
Keywords: Antimicrobial succeptibility, Burn, Pseudomonas aeruginosa, Lahore,

Frequency and antimicrobial sensitivity Pattern of Pseudomonas areuginosa from burn Patients in Lahore, Pakistan

Introduction: Burn injuries are more common in low income countries and are one of the most leading causes of increasing mortality in population. Increasing pattern of drug resistance has made it difficult to treat patients promptly. A descriptive study was planned to estimate the frequency and susceptibility pattern of Pseudomonas aeruginosa in burn patients and was carried out at the Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore during 2014. Materials & Methods: A total of 170  swab samples were collected from 90 indoor patients from burn wound infected patients from Mayo Hospital, Lahore, Pakistan. Samples were then subjected to culture on different microbiological media (MacConkey’s agar, Blood agar, Nutrient agar and Cetrimide agar) alongwith different biochemical tests.  Antimicrobial sensitivity was done with disc diffusion procedure. Results: Revealed 90/170 (52.9%) samples were contaminated with Pseudomonas aeruginosa in 49 (54.45 %) male and 41 (45.56 %) female patients with 31.23 years mean age. The most common cause was flame burns 41(45.55 %), followed by scalds burns 22 (24.44 %), electric burns 15 (16.66 %) and acid burns 12 (13.33 %), respectively. A total 78 isolates of Pseudomonas aeruginosa were recovered from burn patients and most susceptible antibiotic were Imipenem 75.65 %, Cefatoxime 65.39 %, Tobramycin 64.11 % and Amikacin 60.26 % against it. Conclusion: Majority of burn patients were male aging 30-50 Years with flame burn main cause of accident and incident.

Keywords: Antimicrobial succeptibility, Burn, Pseudomonas aeruginosa, Lahore.